Cell Culture Inserts

Industry-leading solutions for cell culturing: precise, reliable, and tailored to your needs

Three cell culture inserts in different sit near a well plate

Cell culturing is a fundamental technique used to study and cultivate human or animal cells in controlled environments. It plays a vital role in various fields, such as biochemistry, molecular biology, and drug testing. By cultivating cells, researchers can gain insights into metabolic processes and assess the effectiveness of drugs, vaccines, and therapeutic proteins.

Cell culture inserts from our sister company, Oxyphen, feature UNIQUE-MEM® track-etched membranes, directly bonded to the insert. This unique combination ensures a strong bond between the plastic body and the smooth membrane surface, making them suitable for large inserts and unconventional cell culture formats.

These customizable track-etched membranes are particularly suitable due to their precise performance parameters. They offer flexibility, allowing you to choose from standard hanging designs or customized inserts based on your specific needs.

Options for customizing cell culture inserts

Excellent cell attachment and growth
Best fluorescence imaging quality
High visibility of particles and cells
Backflushing allows reproducible sample recovery
100% Free of PFOA: Compliant with (EC) 1907/2006 REACH / Regulation (EU) 2019/1021 POP

Rane Material	Pore Size	Application
PET	small 0.4 um	Co-Culturing Drug Transport Studies Secretion Basolateral Diffusion Cytotoxicity studies
PET	large 3.0 – 8.0um	Cell invasion Chemotactic Transport of larger molecules (lipoproteins, viruses) Trans-endothelial migration smooth muscle migration
PC	TBD	Please talk to one our experts for PC as a material option.

Membrane Material	Opacity	Application
PET	Translucent	Electron Microscope TEER
PET	Transparent	Light Microscope Electron Microscope

Membrane Material	# of wells
PET	Multiwell plates (48 / 96 well)
PET	Multiwell plates (48 / 96 well) Cell culture inserts (6 / 12 / 24 well)

Membrane Material

# of wells

PET

Multiwell plates (48 / 96 well)

PET

Multiwell plates (48 / 96 well)

Cell culture inserts (6 / 12 / 24 well)

Air Flow	Up to 800 l / (bar cm2 min)
Pore Size	0.1µm to 10µm
Pore Density	0.01M to 1,000M pores per cm2
Thickness	8µm to 50µm
Temperature Range	– 40°C and 160°C (up to 200°C)
Hydrophilic Treatments	Various hydrophilic treatments increase water flow rate and wetting abilities of the membrane
Sterilization Procedures	Stable against Gamma and X-ray

Specification	Unique-Mem® Track-Etched Membranes	RoTrac® Track-Etched Membranes
Bandwidth (mm)	10, 13, 14.5, 20 & custom sizes
High Quality Raw Materials	PET – naturally slightly hydrophilic PC – naturally slightly hydrophobic	PET-Membrane/ PET-Nonwoven –harder PET-Membrane/ PP-Nonwoven – softer
Pore Size Ranges	0.1µm to 10µm	0.2µm to 3.0µm
Pore Density Ranges	10⁵ to 10⁹ pores per cm²	2×10⁶ bis 3×10⁸ pores per cm²
Thickness Ranges	8µm to 50µm	90µm to 220µm
Temperature Ranges	– 40°C and 160°C (for special application < 200°C)	– 40°C and 130°C (for special application < 160°C)
Air Flow Ranges	Up to 800 l / (bar cm² min)	Up to 37 l / (bar cm² min)
Hydrophilic Treatments	Various hydrophilic treatments increase water flow rate and wetting abilities of the membrane (example: PVP treatment)
Hydrophobic / Oleophobic Treatment:	Grade up to 7.5; AATCC TM 118
Salt spray resistance test²	According to IEC 60068-2-52, based on validation by the customer
Temperature resistance test¹	In accordance with ISO 16750-4
Ice water shock test^1&2	In accordance with ISO 16750-4
Climate resistance test	In accordance with ISO 16750-4
Chemical resistance test¹	According LV124/ ISO 16750-5
IP Protection¹	IP 64/65/66/67/68, according to DIN 40050

¹ Testing in Progress

² Based on Customer Validation

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